PuraMatrix® is the first commercial synthetic extracellular matrix (ECM) scaffold, enabling cells to be cultured in carefully controlled microenvironments for life science research, pharmaceutical drug discovery and bioproduction. PuraMatrix® enhances valuable and expensive cell assays that predict proper drug interactions and metabolism. In particular, PuraMatrix® has improved cell assays in which one or more types of cells form complex structures (such as spheroids, networks, synapses, vascularization). The result is improved models for in vivo and in vitro research and testing, increasing predictability and reducing the cost of drug discovery research. Click here to review PuraMatrix® Guidelines for Use.
Q. Is PuraMatrix® compatible with confocal microscopy and other imaging technologies?
A. Yes. PuraMatrix® forms a clear gel that allows strong resolution for microscopy and radiology applications.
Q. Can PuraMatrix® be stained?
A. PuraMatrix® is transparent, lending many benefits to visualizing the cells and structures growing within the scaffolds. Trypan blue can be used to visualize the scaffolds along with dead cells, which take up the blue stain.
Q. Can molecular analyses of proteins and nucleic acids be performed using cells cultured in PuraMatrix®?
A. Yes. Cells cultured in PuraMatrix® can be prepared for most molecular techniques. Following mechanical disruption of the gel, the cells can be isolated using centrifugation and then processed according to standard procedures.
Q. Is PuraMatrix® compatible with staining and immunodetection protocols?
A. Yes. Cells can be stained with fluorescent dyes and most immunological reagents using standard methods.